Rapid microbial testing in food without PCR - HybriScan® system
Summary
The video introduces a hybrid scan system for rapid microbial quality control in food testing, reducing result time by up to 14 days. False positive results from dead bacteria are explained, along with the highly sensitive in situ sandwich hybridization method used even for crude samples. The workflow for preparing solid food samples and detecting a small number of viable bacterial or yeast cells is detailed, including the process of opening bacterial cell walls to release ribosomal RNA. Additionally, the hybridization plate preparation, enzyme binding process, and interpretation of results via color changes for positive samples are discussed.
Introduction to Hybrid Scan System
Introduction to the hybrid scan system for food testing, a rapid microbial quality control technology based on in situ hybridization that reduces the time to result by two to fourteen days with low costs.
False Positive Results and Sensitivity
Explanation of false positive results from dead bacteria and the highly sensitive in situ sandwich hybridization method used, even for crude samples and not susceptible to matrix interference.
Workflow for Sample Preparation
Description of the workflow for preparing solid food samples using the enrichment sample homogenizer, with the hybrid scan method being very sensitive and requiring only a small number of viable bacterial or yeast cells for detection.
Bacterial Cell Wall Opening and RNA Release
Process of opening bacterial cell walls to release ribosomal RNA using glass beads and lysis buffers, followed by further steps in the release of ribosomal RNA.
Hybridization Plate Preparation
Preparation of the hybridization plate with capture probes, pre-incubation, and subsequent steps for hybridization with the probes.
Coupling Reaction and Enzyme Binding
Description of the coupling reaction to bind the sandwich complex, enzyme binding, and incubation processes to detect the targeted organism using a chromogenic substrate.
Reaction with Chromogenic Substrate
Process of reacting the enzyme with a chromogenic substrate, indicating the presence of the targeted organism, and the color changes for positive samples.
Detection, Confirmation, and Identification
Explanation of the steps for detecting, confirming, and identifying the microorganism concentration in the sample using a microplate reader and interpreting the results based on color intensity.
FAQ
Q: What is the hybrid scan system for food testing?
A: The hybrid scan system is a rapid microbial quality control technology based on in situ hybridization that reduces the time to result by two to fourteen days with low costs.
Q: How does the hybrid scan system address false positive results?
A: False positive results are addressed by the highly sensitive in situ sandwich hybridization method used, even for crude samples, and not susceptible to matrix interference.
Q: What is the workflow for preparing solid food samples with the hybrid scan method?
A: The workflow involves using an enrichment sample homogenizer, with the hybrid scan method being very sensitive and requiring only a small number of viable bacterial or yeast cells for detection.
Q: Can you explain the process of opening bacterial cell walls in the hybrid scan system?
A: The process involves using glass beads and lysis buffers to release ribosomal RNA, followed by further steps in the release of ribosomal RNA.
Q: What are the steps involved in the preparation of the hybridization plate in the hybrid scan system?
A: The steps include preparing the plate with capture probes, pre-incubation, and subsequent hybridization with the probes.
Q: How is the targeted organism detected in the hybrid scan system?
A: The detection is done through a coupling reaction to bind the sandwich complex, enzyme binding, and incubation processes using a chromogenic substrate.
Q: How are positive samples identified in the hybrid scan system?
A: Positive samples are identified by reacting the enzyme with a chromogenic substrate, which leads to color changes indicating the presence of the targeted organism.
Q: What is the final step in detecting and confirming the microorganism concentration in the sample?
A: The final step involves using a microplate reader to interpret the results based on the color intensity of the samples.
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